BAL and total lung cell counts were determined with a haemocytometer, and 1–2 million cells were used for staining. Cells were initially stained with viability dye e506 (Invitrogen, 20 min, 4 °C) and ...

Colony morphology was observed daily, and micromorphology was examined using an optical microscope at 1,000 × magnification after Gram staining. The VITEK 2 Compact ... to induce lysis of the yeast ...

Lamina propria cells from either cecal and colonic or small intestinal tissue were harvested as described. For intracellular cytokine staining, cells were stimulated using the eBioscience cell ...

However, how this dysregulation occurs remains to be elucidated. In this study, we use single-cell RNA sequencing (scRNA-seq) and conventional RNA-seq to analyze the immune landscape of sepsis and ...

The most common causes of IFD are Candida spp., followed by Aspergillus spp.; other pathogens such as Cryptococcus spp., the Mucorales, and Pneumocystis accounting for varying frequency of IFDs ...