Background Gut microbial metabolites are recognised as critical effector molecules that influence the development of colorectal cancer (CRC). Peptidoglycan fragments (PGFs) produced by microbiota play ...

Poorly energized mitochondria trap a subpopulation of mitochondrial precursor proteins in the intermembrane space. This article introduces ‘mitochondrial triage of precursor proteins’ (MitoTraP) as a ...

An aliquot of the subsequent cleared lysate was retained to represent the whole cell fraction and the remainder added to prewashed (in lysis buffer) streptavidin beads (Streptavidin sepharose—Cytiva ...

Understanding weak interactions in protein–ligand complexes is essential for advancing drug design. Here, we combine experimental and quantum mechanical approaches to study the streptavidin–biotin ...

After centrifugation, 500 μl cleared lysate was incubated with 50 μl preequilibrated streptavidin-sepharose beads (Cytiva) with rotation at 4 °C overnight.

AlphaFold2-based large-scale screening reveals novel protein interactions, accelerating functional studies of piRNA pathway proteins and expanding bioinformatic-experimental integration.

Immunoreagents, most commonly antibodies, are integral components of lateral flow immunoassays. However, the use of antibodies comes with limitations, particularly relating to their reproducible ...

Streptavidin Sepharose High Performance (GE Healthcare) was acetylated by the addition of 10 mM sulfo-NHS acetate (Thermo Fisher Scientific) for 30 min twice and then equilibrated in lysis buffer. For ...