Whole genome bisulfite sequencing (WGBS) converts unmethylated cytosine (C) to thymine (T) with the bisulfite treatment on genomic DNA. Subsequently, whole genome resequencing of the treated DNA and ...

After treatment with sodium bisulfite, unmethylated cytosine residues become uracil residues, which are recognized as thymine after PCR amplification and sequencing analysis. Researchers determine ...

Prior to DNA methylation sequencing, researchers treat their samples with sodium bisulfite to distinguish methylated cytosine from unmethylated cytosine. Scientists have discovered that a lack of iron ...

Whole-Genome-Bisulfite Sequencing Whole-Genome Bisulfite Sequencing (WGBS) allows for genome-wide DNA methylation (5-mC) profiling. The Genomic and RNA Profiling core offers a one-stop WGBS workflow ...

Next-generation sequencing technology has made it easier than ever for quick diagnosis of plant diseases. “It’s really exciting to see how sequencing technologies have evolved and how this new ...

Efforts by a team of scientists working around the world have paid off. They have succeeded in completing the first genetic map of a plant. Completion of the sequencing of Arabidopsis thaliana, a ...

Another limitation of bisulfite sequencing is that it detects 5mC and 5hmC only indirectly. The technique converts unmodified cytosine to a related base called uracil, while leaving methylated ...

When sequencing DNA using a single-base coding system with a four-state readout (G, C, T, and A), you can report on a maximum of four genetic or epigenetic information states in a single run.

Bisulfite sequencing is widely used to profile epigenetic DNA marks at single-base resolution, but it cannot distinguish between 5-methylcytosine (5-mC), a common repressive modification, and the ...

Farlik, M. et al. Cell Rep. 10, 1386–1397 (2015). Treating DNA with bisulfite before sequencing enables the detection of methylated cytosine residues across the genome.