Cytosine has the chemical formula C 4 H 5 N 3 O and a molar mass of 111.10 g/mol. It is a weakly basic compound due to the presence of the primary amine group. The heterocyclic aromatic ring of ...

After treatment with sodium bisulfite, unmethylated cytosine residues become uracil residues, which are recognized as thymine after PCR amplification and sequencing analysis. Researchers determine ...